Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB29555 - Provider product page
- Provider
- Abnova Corporation
- Product name
- MAP1LC3A polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant human MAP1LC3A.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Nontransfected 293 cell lysates (2 ug/lane), Lane 2: Transiently transfected 293 cell lysates (2 ug/lane) with MAP1LC3A polyclonal antibody (Cat # PAB29555) at 1:1000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Chloroquine (50 uM, 16h) treated U251 cells with MAP1LC3A polyclonal antibody (Cat # PAB29555) shows positivity in cytoplasmic autophagic vacuoles at 1:200 dilution.
- Validation comment
- Immunofluorescence