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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- F48472 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Phospho-LC3C Antibody (pS12)
- Antibody type
- Polyclonal
- Antigen
- This phospho-LC3C antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS12 of human LC3C.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the phospho-LC3C antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- WB: Wild type LC3 and LC3 S12A mutant vectors were transfected into CHO cells and tested with phospho-LC3C antibody (S12A = replacement of the amino acid position 12 serine of LC3 with alanine). Expected size: LC3-I = 16kDa, and LC3-II = 14 kDa
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- WB: Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with phospho-LC3C antibody. The data shows that treatment with rapamycin showed no significant change in level of LC3.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- WB: Immunoblots of SH-SY5Y cells treated with MPP+ for 24h was probed with phospho-LC3C antibody. The data shows that treatment with MPP+ showed no significant change in level of LC3.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SH-SY5Y cells expressing GFP-LC3-WT or-S12D mutation (reduced puncta) treated with rapamycin or vehicle for 1h and probed with phospho-LC3C antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Dot blot analysis of phospho-LC3C antibody and nonphos Ab. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
