Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARG55622 - Provider product page
- Provider
- Arigo
- Product name
- anti-LC3 antibody
- Antibody type
- Monoclonal
- Antigen
- Full length recombinant protein of Human LC3.
- Description
- Purification with Protein G.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 166AT1234
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: HeLa cell lysates treated with rapamycin or bafilomycin overnight, stained with ARG55622 anti-LC3 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 1) Y79 (soluble fraction of cell extract) and 2) 293 transfected with human LC3 (whole cell extract) stained with ARG55622 anti-LC3 antibody at 8 ug/ml dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cells stained with ARG55622 anti-LC3 antibody (green) at 1:200 dilution, 2 h at room temperature. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ARG55622 anti-LC3 antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cells stained with ARG55622 anti-LC3 antibody (green) at 1:25 dilution. DAPI was used to stain the cell nuclear (blue).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cells stained with ARG55622 anti-LC3 antibody (green) at 1:25 dilution. DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: 10X (lower panel) and 20X (upper panel) muscle tissue of a diseased mouse off Dox after 5 weeks on regular food, stained with ARG55622 anti-LC3 antibody. Several fibers that have autophagic vesicles throughout are visible.