Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- ABIN1449607 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (AA 501-531) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 501~531 amino acids surrounding amino acid R509 of Human APG7L Genename: ATG7
- Description
- Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 501-531
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line.
Energy restriction as an antitumor target of thiazolidinediones.
Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase.
A novel protein complex in membrane rafts linking the NR2B glutamate receptor and autophagy is disrupted following traumatic brain injury.
A plant triterpenoid, avicin D, induces autophagy by activation of AMP-activated protein kinase.
Okamoto K, Sakimoto Y, Imai K, Senoo H, Shidoji Y
The Biochemical journal 2011 Nov 15;440(1):63-71
The Biochemical journal 2011 Nov 15;440(1):63-71
Energy restriction as an antitumor target of thiazolidinediones.
Wei S, Kulp SK, Chen CS
The Journal of biological chemistry 2010 Mar 26;285(13):9780-91
The Journal of biological chemistry 2010 Mar 26;285(13):9780-91
Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase.
Yang D, Liu H, Goga A, Kim S, Yuneva M, Bishop JM
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 3;107(31):13836-41
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 3;107(31):13836-41
A novel protein complex in membrane rafts linking the NR2B glutamate receptor and autophagy is disrupted following traumatic brain injury.
Bigford GE, Alonso OF, Dietrich D, Keane RW
Journal of neurotrauma 2009 May;26(5):703-20
Journal of neurotrauma 2009 May;26(5):703-20
A plant triterpenoid, avicin D, induces autophagy by activation of AMP-activated protein kinase.
Xu ZX, Liang J, Haridas V, Gaikwad A, Connolly FP, Mills GB, Gutterman JU
Cell death and differentiation 2007 Nov;14(11):1948-57
Cell death and differentiation 2007 Nov;14(11):1948-57
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis using ATG7 Antibody Cat.-No AP32175PU-N to detect APG7L in Mouse liver tissue lysate.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis using ATG7 Antibody Cat.-No AP32175PU-N (1/60 dilution) in 293 cell line lysates transiently transfected with the ATG7 gene (2 μg/Lane).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.