H00007188-D01P

antibody from Abnova Corporation

Targeting: TRAF5 RNF84

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007188-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007188-D01P, RRID:AB_1581472

Product name

TRAF5 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human TRAF5 protein.

Antigen sequence

MAYSEEHKGMPCGFIRQNSGNSISLDFEPSIEYQF
VERLEERYKCAFCHSVLHNPHQTGCGHRFCQHCIL
SLRELNTVPICPVDKEVIKSQEVFKDNCCKREVLN
LYVYCSNAPGCNAKVILGRYQDHLQQCLFQPVQCS
NEKCREPVLRKDLKEHLSASCQFRKEKCLYCKKDV
VVINLQNHEENLCPEYPVFCPNNCAKIILKTEVDE
HLAVCPEAEQDCPFKHYGCAVTDKRRNLQQHEHSA
LREHMRLVLEKNVQLEEQISDLHKSLEQKESKIQQ
LAETIKKLEKEFKQFAQLFGKNGSFLPNIQVFASH
IDKSAWLEAQVHQLLQMVNQQQNKFDLRPLMEAVD
TVKQKITLLENNDQRLAVLEEETNKHDTHINIHKA
QLSKNEERFKLLEGTCYNGKLIWKVTDYKMKKREA
VDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGR
GSHLSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQS
GKKNIMETFKPDPNSSSFKRPDGEMNIASGCPRFV
AHSVLENAKNAYIKDDTLFLKVAVDLTDLEDL


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TRAF5 expression in transfected 293T cell line (H00007188-T01) by TRAF5 MaxPab polyclonal antibody.Lane 1: TRAF5 transfected lysate(64.40 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between TRAF5 and TRAF3. HeLa cells were stained with anti-TRAF5 rabbit purified polyclonal 1:1200 and anti-TRAF3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)