PA5-21680

antibody from Invitrogen Antibodies

Targeting: NCKAP1 HEM2, Nap1, NAP125

Antibody data

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Validation data

Product number: PA5-21680 - Provider product page
Provider: Invitrogen Antibodies
Product name: NCKAP1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: NCKAP1-transfected 293T, Mouse brain, rat brain.
Concentration: 0.5 mg/mL

NCKAP1 protein structure - PA5-21680 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NCKAP1 using 30µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NCKAP1 polyclonal antibody (Product # PA5-21680) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of NCKAP1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NCKAP1 Polyclonal Antibody (Product # PA5-21680) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using NCKAP1 Polyclonal Antibody (Product # PA5-21680). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 5% SDS PAGE. NCKAP1 Polyclonal Antibody (Product # PA5-21680) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NCKAP1 Polyclonal Antibody detects NCKAP1 protein by Western blot analysis. A. 50 µg rat brain lysate/extract.5 % SDS-PAGE. NCKAP1 Polyclonal Antibody (Product # PA5-21680) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-NCKAP1 Rabbit Polyclonal Antibody (Product # PA5-21680) and a 130kDa band corresponding to NCKAP1 was observed across cell lines and tissues tested except U-937 and HL-60 which are reported negative for NCKP1 expression. An uncharacterized band was also observed at ~30kDa in certain cell lines. Membrane enriched extracts (30 µg lysate) of U-87 MG (Lane 1), NTERA-2 cl.D1 (Lane 2), A-431 (Lane 3), U-2 OS (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6), U-937 (Lane 7) and HL-60 (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).