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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-29651 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Collagen I alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This Collagen I alpha 1 antibody is specific for the propeptide portion of the ~180 kDa collagen I alpha1 polypeptide found in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Collagen I alpha 1 Antibody [NBP2-29651] - Rat lung lysate showing specific immunolabeling of collagen I alpha 1 with observed molecular weight at ~180.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen I alpha 1 Antibody [NBP2-29651] - Collagen I Antibody [NBP2-29651] - fibrotic mouse lung tissue showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized.
