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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-46875 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Collagen I alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards
- Reactivity
- Human, Mouse, Rat, Bovine, Feline, Porcine, Rabbit
- Host
- Rabbit
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Collagen I alpha 1 Antibody [HRP] [NBP2-46875] - Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg transduced cell lysates. Load: 100 ug per lane
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen I alpha 1 Antibody [HRP] [NBP2-46875] - Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Primary antibody: Collagen Type I antibody at 10 ug/mL for 1 h at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).
