668505

antibody from BioLegend

Targeting: GTF2B TFIIB

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

668505

Provider

BioLegend

Proper citation

BioLegend Cat#668505, RRID:AB_2566350

Product name

Alexa Fluor® 594 anti-TFIIB

Antibody type

Monoclonal

Antigen

Recombinant protein.

Description

TFIIB is one of the ubiquitous transcription factors required for transcription initiation by RNA polymerase II. It plays an important role in pre-initiation complex assembly, which provides a bridge between promoter-bound TFIID and RNA polymerase II. Moreover, TFIIB also directly modulates the catalytic center of RNA polymerase II.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

IIB1

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/ml Alexa Fluor® 594 conjugated (red) TFIIB Antibody (clone IIB1) in blocking buffer overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye