GTX48803

antibody from GeneTex

Targeting: TGFBI BIGH3, CDB1, CDGG1, CSD1, CSD2, CSD3, LCD1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX48803

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48803, RRID:AB_11174206

Product name

TGFBI antibody [k1H12]

Antibody type

Monoclonal

Host

Mouse

Vial size

100 µl

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot showing detection of 0.1 µg of recombinant OspB protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspB fusion protein (arrow; expected MW = 72.7 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4°C, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

Validation comment

WB