PA5-27261
antibody from Invitrogen Antibodies
Targeting: TGFBI
BIGH3, CDB1, CDGG1, CSD1, CSD2, CSD3, LCD1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-27261 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TGFBI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TGFBI Polyclonal Antibody (Product # PA5-27261). Sample (30 µg of whole cell lysate). Lane A: HeLa. 7.5% SDS PAGE. TGFBI antibody. TGFBI Polyclonal Antibody (Product # PA5-27261) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-TGFBI Polyclonal Antibody (Product # PA5-27261) and a 68 kDa band corresponding to Transforming growth factor-beta-induced protein ig-h3 was observed across across the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), IMR-32 (Lane 3), Raji (Lane 4), Jurkat (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).