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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00007957-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007957-M01, RRID:AB_1112239
- Product name
- EPM2A monoclonal antibody (M01), clone 4A12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant EPM2A.
- Antigen sequence
GNGPHHDRCCTYNENNLVDGVYCLPIGHWIEATGH
TNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCP
RQVEHVTIKLKHELGITAVMNFQTEWDIV- Isotype
- IgG
- Antibody clone number
- 4A12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references A bioassay for Lafora disease and laforin glucan phosphatase activity.
Sherwood AR, Johnson MB, Delgado-Escueta AV, Gentry MS
Clinical biochemistry 2013 Dec;46(18):1869-76
Clinical biochemistry 2013 Dec;46(18):1869-76
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EPM2A monoclonal antibody (M01), clone 4A12. Western Blot analysis of EPM2A expression in PC-12 ( Cat # L012V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged EPM2A is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
