12-1528-41

antibody from Invitrogen Antibodies
Targeting: CTLA4 CD, CD152, CELIAC3, CTLA-4, GSE, IDDM12
Provider product page for 12-1528-41

Antibody data

Product number
12-1528-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD152 (CTLA-4) Monoclonal Antibody (eBio20A (20A, A3)), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The eBio20A monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells (activated B cells may also express CTLA-4) and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling. Applications Reported: This eBio20A (20A, A3) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio20A (20A, A3) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of PHA stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
eBio20A (20A, A3)
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CTLA4 protein structure - 12-1528-41 shown in red.