Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [5]
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Validation data
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- Product number
- LS-C742105 - Provider product page
- Provider
- LSBio
- Product name
- CTLA4 / CD152 Antibody (clone CT.E8) LS-C742105
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CT.E8
- Storage
- Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA blocking of human CTLA4 antibody CT.E8 against Human CD86 recombinant protein (141-B2, CD86 Fc Chimera, Human) binding with Human CTLA4 recombinant protein (7268-CT, CTLA4 Fc Chimera, Human). Coating antigen: PD-1-Fc, 1 µg/ml. CD86-Fc final concentration: 0.5 µg /ml CTLA4 antibody dilution start from 50 µg/ml, IC50= 0.112 µg/ml
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA binding of human CTLA4 antibody CT.E8 with Human CTLA4 recombinant protein (CTLA4 Fc Chimera, Human). Coating antigen: CTLA4-Fc, 0.5 µg/ml. CTLA4 antibody dilution start from 10000 ng/ml, EC50= 4.66 ng/ml
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA binding of human CTLA4 antibody CT.E8 with Human CTLA4 recombinant protein (CTLA4 Fc Chimera, Human). Coating antigen: CTLA4-Fc, 0.5 µg/ml. CTLA4 antibody dilution start from 10000 ng/ml, EC50= 4.66 ng/ml
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA blocking of human CTLA4 antibody CT.E8 against Human CD86 recombinant protein (141-B2, CD86 Fc Chimera, Human) binding with Human CTLA4 recombinant protein (7268-CT, CTLA4 Fc Chimera, Human). Coating antigen: PD-1-Fc, 1 µg/ml. CD86-Fc final concentration: 0.5 µg /ml CTLA4 antibody dilution start from 50 µg/ml, IC50= 0.112 µg/ml
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA binding of human CTLA4 antibody CT.E8 with Human CTLA4 recombinant protein (CTLA4 Fc Chimera, Human). Coating antigen: CTLA4-Fc, 0.5 µg/ml. CTLA4 antibody dilution start from 10000 ng/ml, EC50= 4.66 ng/ml