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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347226 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal JMJD2a Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal JMJD2a Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KDM4A
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 0.66 ug/ul
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibody against JMJD2a diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human JMJD2a, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.
- Validation comment
- ELISA
