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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [9]
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- Product number
- PA5-55319 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DBT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VGKPLVDIET EALKDSEEDV VETPAVSHDE HTHQEIKGRK TLATPAVRRL AMENNIKLSE VVGSGKDGRI LKEDILNYLE KQ
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DBT in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DBT Polyclonal Antibody (Product # PA5-55319).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DBT in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a DBT Polyclonal Antibody (Product # PA5-55319).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DBT in human cell line A-431 shows positivity in mitochondria. Samples were probed using a DBT Polyclonal Antibody (Product # PA5-55319).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DBT in human colon using DBT Polyclonal Antibody (Product # PA5-55319).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DBT in human liver using DBT Polyclonal Antibody (Product # PA5-55319).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DBT in human lymph node using DBT Polyclonal Antibody (Product # PA5-55319).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DBT in human kidney and pancreas tissues using DBT Polyclonal Antibody (Product # PA5-55319). Corresponding DBT RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DBT in human colon, liver, lymph node and pancreas using DBT Polyclonal Antibody (Product # PA5-55319) (A) shows similar protein distribution across tissues to an independent DBT Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DBT in human prostate using DBT Polyclonal Antibody (Product # PA5-55319) shows weak to moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DBT in human testis using DBT Polyclonal Antibody (Product # PA5-55319) shows weak to moderate cytoplasmic positivity in Leydig cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DBT in human kidney using DBT Polyclonal Antibody (Product # PA5-55319) shows moderate to strong cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DBT in human pancreas using DBT Polyclonal Antibody (Product # PA5-55319) shows weak to moderate cytoplasmic positivity in exocrine glandular cells.
