AB-416-NA

antibody from Novus Biologicals

Targeting: CSF1 M-CSF, MCSF, MGC31930

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-416-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal M-CSF Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) M-CSF is observed. Neutralizes the biological activity of recombinant mouse M-CSF and will also neutralize the biological activity of recombinant human M-CSF at a 100 fold higher IgG concentration.

Reactivity

Mouse

Host

Goat

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

IL-34 is a Treg-specific cytokine and mediates transplant tolerance.

Bézie S, Picarda E, Ossart J, Tesson L, Usal C, Renaudin K, Anegon I, Guillonneau C
The Journal of clinical investigation 2015 Oct 1;125(10):3952-64

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by M-CSF and Neutralization by Mouse M-CSF Antibody. Recombinant Mouse M-CSF (Catalog # 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Mouse M-CSF Polyclonal Antibody (Catalog # AB-416-NA). The ND₅₀ is typically 0.2-0.6 µg/mL.