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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB18181 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DLL1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human DLL1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DLL3 or rhDLL4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAG09716- Isotype
- IgG
- Antibody clone number
- 251123
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Hypoxia-induced Jagged2 promotes breast cancer metastasis and self-renewal of cancer stem-like cells.
Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.
Xing F, Okuda H, Watabe M, Kobayashi A, Pai SK, Liu W, Pandey PR, Fukuda K, Hirota S, Sugai T, Wakabayshi G, Koeda K, Kashiwaba M, Suzuki K, Chiba T, Endo M, Mo YY, Watabe K
Oncogene 2011 Sep 29;30(39):4075-86
Oncogene 2011 Sep 29;30(39):4075-86
Influence of the Notch system in the therapeutic response of American tegumentary leishmaniasis.
Rodrigues KM, Oliveira MP, Maretti-Mira AC, Oliveira-Neto MP, Mattos MS, Silva L, Soares DA, Dolci EL, Perico RA, Pirmez C
The British journal of dermatology 2011 Jun;164(6):1228-34
The British journal of dermatology 2011 Jun;164(6):1228-34
Notch activation differentially regulates renal progenitors proliferation and differentiation toward the podocyte lineage in glomerular disorders.
Lasagni L, Ballerini L, Angelotti ML, Parente E, Sagrinati C, Mazzinghi B, Peired A, Ronconi E, Becherucci F, Bani D, Gacci M, Carini M, Lazzeri E, Romagnani P
Stem cells (Dayton, Ohio) 2010 Sep;28(9):1674-85
Stem cells (Dayton, Ohio) 2010 Sep;28(9):1674-85
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DLL1 in HepG2 Human Cell Line. DLL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB18181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
