Explore
Validate
Learn
Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN2616891 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Adipocyte Plasma Membrane Associated Protein (APMAP) (AA 1-416) antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 1-416
- Isotype
- IgG
- Vial size
- 50 μg
- Storage
- May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
- Handling
- Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- EZH2 antibody (mAb) tested by ChIP. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 μg of EZH2 antibody or the equivalent amount of IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from the ChIP reaction using a primer pair specific for the Myt1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
