Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-34748 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP19 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: human synovial fluid, MMP19-transfected 293T.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of MMP19 using 30 µg of U87-MG lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MMP19 polyclonal antibody (Product # PA5-34748) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of MMP19 was performed by separating 30 µg of human synovial fluid extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a MMP19 Polyclonal Antibody (Product # PA5-34748) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MMP19 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a MMP19 Polyclonal Antibody (Product # PA5-34748) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MMP19 Polyclonal Antibody detects MMP19 protein at extracellular matrix on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MMP19 Polyclonal Antibody (Product # PA5-34748) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.