330902

antibody from BioLegend

Targeting: CD58 LFA3

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

330902

Provider

BioLegend

Proper citation

BioLegend Cat#330902, RRID:AB_1186186

Product name

Purified anti-human CD58 (LFA-3)

Antibody type

Monoclonal

Antigen

Human cytolytic T cells

Description

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a 45-70 kD cell surface protein that is a member of the immunoglobulin superfamily. Alternative splicing of CD58 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on antigen presenting cells and target cells enhances T cell recognition via the binding of it's cognate ligand, CD2, on the T cell surface. The HCD58 antibody recognizes human CD58 and has been shown to be useful for flow cytometry.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes stained with purified TS2/9, followed by anti-mouse IgG FITC

Conjugate

Unconjugated