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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- NBP1-77990 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77990, RRID:AB_11007022
- Product name
- Rabbit Polyclonal SETDB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Western blotting shows reactivity specific for human ESET detecting a band at approximately 170 kDa. Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.17 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly.
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.
SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins.
Sarraf SA, Stancheva I
Molecular cell 2004 Aug 27;15(4):595-605
Molecular cell 2004 Aug 27;15(4):595-605
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.
Wang H, An W, Cao R, Xia L, Erdjument-Bromage H, Chatton B, Tempst P, Roeder RG, Zhang Y
Molecular cell 2003 Aug;12(2):475-87
Molecular cell 2003 Aug;12(2):475-87
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.
Wang H, An W, Cao R, Xia L, Erdjument-Bromage H, Chatton B, Tempst P, Roeder RG, Zhang Y
Molecular cell 2003 Aug;12(2):475-87
Molecular cell 2003 Aug;12(2):475-87
SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins.
Schultz DC, Ayyanathan K, Negorev D, Maul GG, Rauscher FJ 3rd
Genes & development 2002 Apr 15;16(8):919-32
Genes & development 2002 Apr 15;16(8):919-32
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: SETDB1 Antibody [NBP1-77990] - detect human ESET present in a 293 whole cell lysate. ~30ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min.
