Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA807098 - Provider product page
- Provider
- OriGene
- Product name
- PD-1 (PDCD1) mouse monoclonal antibody, clone OTI26C4
- Antibody type
- Monoclonal
- Description
- PD-1 (PDCD1) mouse monoclonal antibody, clone OTI26C4
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDCD1
- Isotype
- IgG
- Antibody clone number
- OTI26C4
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1.(1:2000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA807098 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA807098)(Left) . The rihgt is negative control.(1:100)(1:900)
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of stable expression PDL1(RC213071) cells using anti-PDCD1 antibody(TA807098) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red),and detected by anti-Fc(human) IgG-FITC.(1:50)
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody(TA807098) (Red) compared to a nonspecific negative control antibody (Blue).(1:50)
- Validation comment
- FC
