Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Flow cytometry [4]
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Validation data
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- Product number
- NBP1-43110-0.5mg - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-43110-0.5mg, RRID:AB_10006455
- Product name
- Armenian Hamster Monoclonal PD-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Mouse
- Isotype
- IgG
- Vial size
- 0.5 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster Ig
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Biotin conjugate of NBP1-43110. Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis of PE conjugate of NBP1-43110. The J43 antibody has been tested by flow cytometric mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be de