- PA5-30583 - Invitrogen Antibodies LORICRIN antibody

Girousi E, Muerner L, Parisi L, Rihs S, von Gunten S, Katsaros C, Degen M
Frontiers in cell and developmental biology 2021;9:718066

Yao Q, Jia T, Qiao W, Gu H, Kaku K
Journal of cosmetic dermatology 2021 Jan;20(1):321-329

Degen M, Girousi E, Feldmann J, Parisi L, La Scala GC, Schnyder I, Schaller A, Katsaros C
Frontiers in cell and developmental biology 2020;8:583115

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Loricrin using 30 µg of Raji lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Loricrin polyclonal antibody (Product # PA5-30583) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Loricrin in whole cell extract (30 µg). Samples was separated by 12% SDS-PAGE and the membrane was probed with Loricrin, N-term Polyclonal antibody (Product # PA5-30583) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of LOR was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a LOR Polyclonal Antibody (Product # PA5-30583) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using LOR Polyclonal Antibody (Product # PA5-30583). Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with LOR Polyclonal Antibody (Product # PA5-30583) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-LOR Polyclonal Antibody (Product # PA5-30583) and a 26kDa band corresponding to LOR was observed in Mouse Skin and not in Mouse Heart or Mouse Lung along with an uncharacterized band (*) at ~45 kDa in Mouse Heart and at ~60 kDa in Mouse Lung. Tissue extracts (30 µg lysate) of Mouse Skin (Lane 1), Mouse Heart (Lane 2), and Mouse Lung (Lane 3) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Loricrin in methanol-fixed Hep3B cells using a Loricrin polyclonal antibody (Product # PA5-30583) at a 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: LOR Polyclonal Antibody detects LOR protein at cytosol on HBL438 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL438 xenograft. LOR Polyclonal Antibody (Product # PA5-30583) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 5 (A) Live Imaging pictures and F-actin staining (phalloidin, red) with or without (only Live Imaging) exogenous Ca 2+ addition. While the control keratinocytes start to differentiate (asterisk and arrowheads), lack of IRF6 impairs differentiation. Scale bar: 200 mum (Live Imaging); Scale bar: 150 mum (F-actin). (B) Heatmaps of the qPCR analyses of various differentiation markers in basal (0.1 mM) vs. high Ca 2+ (1.2 mM) conditions. Note that in the absence of IRF6 all the differentiation markers are not induced upon the Ca 2+ -switch. n.d. : not detectable (Ct > 32); ctrl. : control. (C) qPCR analyses of specific differentiation markers showing the lack of induction upon the addition of Ca 2+ in the IRF6 knockout clones compared to controls. * p < 0.05 basal vs. 1.2 mM Ca 2+ . ctrl. : control. (D) Immunofluorescent staining for the markers Involucrin (IVL, green) and Transglutaminase 1 (TGM1, green) in OKF6/TERT2 cells and for IVL (green) and Loricrin (LOR, green) in N/TERT1 keratinocytes. Note that all differentiation markers were robustly induced in the control cells in the presence of IRF6. Scale bar: 50 mum. DAPI was used to counterstain the cell nuclei (blue).

PA5-30583