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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 18539-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SH2D2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEFPLAQIC PQGSHEAPIP TFSTFQITDM TRRSCQNLGY TAASPQAPEA ASSTGNAERA EEVPGEGSLF LQAETRAWFQ KTQAHWLLQH GAAPAWFHGF ITRREAERLL EPKPQGCYLV RFSESAVTFV LTYRSRTCCR HFLLAQLRDG RHVVLGEDSA HARLQDLLLH YTAHPLSPYG ETLTEPLARQ TPEPAGLSLR TEESNFGSKS QDPNPQYSPI IKQGQAPVPM QKEGAGEKEP SQLLRPKPPI PAKPQLPPEV YTIPVPRHRP APRPKPSNPI YNEPDEPIAF YAMGRGSPGE A (1-300 aa encoded by BC012107)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.57 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 18539-1-AP (SH2D2A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 18539-1-AP (SH2D2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 18539-1-AP (SH2D2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 18539-1-AP (SH2D2A antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 K-562 cells were stained with 0.2ug SH2D2A antibody (18539-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
