Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 13-5899-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions. Applications Reported: AER-37 has been reported for use in flow cytometric analysis. Applications Tested: The AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- AER-37 (CRA1)
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria.
IgE- and FcepsilonRI-mediated migration of human basophils.
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Fujisawa D, Kashiwakura J, Kita H, Kikukawa Y, Fujitani Y, Sasaki-Sakamoto T, Kuroda K, Nunomura S, Hayama K, Terui T, Ra C, Okayama Y
The Journal of allergy and clinical immunology 2014 Sep;134(3):622-633.e9
The Journal of allergy and clinical immunology 2014 Sep;134(3):622-633.e9
IgE- and FcepsilonRI-mediated migration of human basophils.
Suzukawa M, Hirai K, Iikura M, Nagase H, Komiya A, Yoshimura-Uchiyama C, Yamada H, Ra C, Ohta K, Yamamoto K, Yamaguchi M
International immunology 2005 Sep;17(9):1249-55
International immunology 2005 Sep;17(9):1249-55
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Yamaguchi M, Sayama K, Yano K, Lantz CS, Noben-Trauth N, Ra C, Costa JJ, Galli SJ
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.125 µg of Mouse IgG2b Isotype Control Biotin (Product # 13-4732-85) (open histogram) or 0.06 µg of Anti-Human Fc epsilon Receptor I alpha (Fc epsilon R1) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Biotin