PA5-14072

antibody from Invitrogen Antibodies

Targeting: STK11 LKB1, PJS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14072

Provider

Invitrogen Antibodies

Product name

LKB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with chicken, mouse, rat and Xenopus based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of STK11 (arrow) using a STK11 polyclonal antibody (Product # PA5-14072) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the STK11 gene (Lane 2).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a STK11 polyclonal antibody (Product # PA5-14072) in T47D cell lysates (35 µg per lane).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ZR-75-1 cells using a STK11 polyclonal antibody (Product # PA5-14072) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a STK11 polyclonal antibody (Product # PA5-14072), followed by HRP-conjugated secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of NCI-H460 cells using a STK11 polyclonal antibody (Product # PA5-14072) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody