AF762

antibody from R&D Systems

Targeting: TEK CD202b, TIE-2, TIE2, VMCM, VMCM1

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF762

Provider

R&D Systems

Product name

Mouse/Rat Tie-2 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse and rat Tie-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) Tie-2 is observed and less than 1% cross-reactivity with recombinant zebrafish Tie-2 and rhTie-1 is observed.

Reactivity

Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

CAA47857


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration.

Kim J, Park JR, Choi J, Park I, Hwang Y, Bae H, Kim Y, Choi W, Yang JM, Han S, Chung TY, Kim P, Kubota Y, Augustin HG, Oh WY, Koh GY
Science advances 2019 Feb;5(2):eaau6732

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Pulmonary pericytes regulate lung morphogenesis.

Kato K, Diéguez-Hurtado R, Park DY, Hong SP, Kato-Azuma S, Adams S, Stehling M, Trappmann B, Wrana JL, Koh GY, Adams RH
Nature communications 2018 Jun 22;9(1):2448

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Tie2 protects the vasculature against thrombus formation in systemic inflammation.

Higgins SJ, De Ceunynck K, Kellum JA, Chen X, Gu X, Chaudhry SA, Schulman S, Libermann TA, Lu S, Shapiro NI, Christiani DC, Flaumenhaft R, Parikh SM
The Journal of clinical investigation 2018 Apr 2;128(4):1471-1484

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Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma.

Kim J, Park DY, Bae H, Park DY, Kim D, Lee CK, Song S, Chung TY, Lim DH, Kubota Y, Hong YK, He Y, Augustin HG, Oliver G, Koh GY
The Journal of clinical investigation 2017 Oct 2;127(10):3877-3896

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Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling.

Morooka N, Futaki S, Sato-Nishiuchi R, Nishino M, Totani Y, Shimono C, Nakano I, Nakajima H, Mochizuki N, Sekiguchi K
Circulation research 2017 Apr 14;120(8):1276-1288

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Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.

Zhou Y, Williams J, Smallwood PM, Nathans J
PloS one 2015;10(12):e0143650

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Endothelial destabilization by angiopoietin-2 via integrin β1 activation.

Hakanpaa L, Sipila T, Leppanen VM, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
Nature communications 2015 Jan 30;6:5962

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COMP-angiopoietin-1 recovers molecular biomarkers of neuropathy and improves vascularisation in sciatic nerve of ob/ob mice.

Kosacka J, Nowicki M, Klöting N, Kern M, Stumvoll M, Bechmann I, Serke H, Blüher M
PloS one 2012;7(3):e32881

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Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway.

Bai Y, Cui M, Meng Z, Shen L, He Q, Zhang X, Chen F, Xiao J
Biochemical and biophysical research communications 2009 Jan 9;378(2):296-301

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Immunotherapy of tumors with protein vaccine based on chicken homologous Tie-2.

Luo Y, Wen YJ, Ding ZY, Fu CH, Wu Y, Liu JY, Li Q, He QM, Zhao X, Jiang Y, Li J, Deng HX, Kang B, Mao YQ, Wei YQ
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Mar 15;12(6):1813-9

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A neurovascular niche for neurogenesis after stroke.

Ohab JJ, Fleming S, Blesch A, Carmichael ST
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Dec 13;26(50):13007-16