14-2029-82

antibody from Invitrogen Antibodies

Targeting: TEK CD202b, TIE-2, TIE2, VMCM, VMCM1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

14-2029-82

Provider

Invitrogen Antibodies

Product name

CD202b (TIE2) Monoclonal Antibody (33.1), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This 33.1 monoclonal antibody reacts with human, mouse, rabbit, rat, pig, and zebrafish CD202b. Also known as Tie-2, this molecule is a member of the tyrosine kinase receptor family. CD202b is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers-, c-Kit+, Sca1+ cells. Long-term multilineage repopulating cells were detected in Tie-2+, Lineage-, c-Kit+, Sca1+ cells but not in Tie-2-, Lineage-, c-Kit+, Sca1+ cells.

Antibody clone number

33.1

Concentration

0.5 mg/mL

References

Submitted references

Angiopoietin 2 stimulates TIE2-expressing monocytes to suppress T cell activation and to promote regulatory T cell expansion.

Coffelt SB, Chen YY, Muthana M, Welford AF, Tal AO, Scholz A, Plate KH, Reiss Y, Murdoch C, De Palma M, Lewis CE
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4183-90

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Angiopoietin-1-induced ubiquitylation of Tie2 by c-Cbl is required for internalization and degradation.

Wehrle C, Van Slyke P, Dumont DJ
The Biochemical journal 2009 Oct 12;423(3):375-80

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Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2.

Nguyen VP, Chen SH, Trinh J, Kim H, Coomber BL, Dumont DJ
BMC cell biology 2007 Mar 6;8:10

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Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver.

Hsu HC, Ema H, Osawa M, Nakamura Y, Suda T, Nakauchi H
Blood 2000 Dec 1;96(12):3757-62

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using CD202b (TIE2) Monoclonal Antibody (33.1), eBioscience™ (Product # 14-2029-82) and a 140 kDa band corresponding to TEK/TIE2 was observed across cell lines and tissues tested except U-87 MG and Mouse adipose which are reported to be negative. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HUVEC (Lane 2), Mouse lung (Lane 3), Mouse kidney (Lane 4), Mouse adipose (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (14-2029-82,1:1000 dilution) and detected by Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).