NBP2-76329

antibody from Novus Biologicals

Targeting: ATF6 ATF6A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-76329

Provider

Novus Biologicals

Product name

Rabbit Monoclonal ATF6 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATF6 Antibody [NBP2-76329] - Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, A20 mouse B cell lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.05 ug/mL of Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (Catalog # NBP2-76329) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ATF6 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: ATF6 Antibody (2358C) [NBP2-76329] - ATF6 was detected in immersion fixed paraffin-embedded sections of human prostate using Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (Catalog # NBP2-76329) at 0.3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: ATF6 Antibody [NBP2-76329] - MCF-7 human breast cancer cell line was stained with Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (Catalog # NBP2-76329, filled histogram) or Rabbit IgG control antibody (Catalog # MAB1050, open histogram) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).