NB100-60666

antibody from Novus Biologicals

Targeting: PTPN12 PTP-PEST, PTPG1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-60666

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-60666, RRID:AB_2269199

Product name

Rabbit Polyclonal PTPN12 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PTPN12 Antibody [NB100-60666] - Detection of Human and Mouse PTPN12/PTP-PEST on HeLa whole cell lysate using NB100-60666. PTPN12/PTPPEST was also IPed by NB100-60663, NB100-60664, and NB100-60665.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PTPN12 Antibody [NB100-60666] - Detection of Human and Mouse PTPN12 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PTPN12 antibody NB100-60666 used at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PTPN12 Antibody [NB100-60666] - Sample: FFPE section of mouse renal cell carcinoma. Antibody used at a dilution of 1:250.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PTPN12 Antibody [NB100-60666] - Sample: FFPE section of human colon carcinoma. Antibody used at a dilution of 1:250.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: PTPN12 Antibody [NB100-60666] - Sample: FFPE section of human stomach carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- PTPN12 used at a dilution of 1:200 (1ug/ml). Detection: DAB