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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [4]
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Validation data
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- Product number
- PA5-95929 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RGS4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: MCKGLAGLPA SCLRSAKDMK HRLGFLLQKS DSCEHNSSHN KKDKVVICQR VSQEEVKKWA ESLENLISHE CGLAAFKAFL KSEYSEENID FWISCEEYKK
- Concentration
- 0.47 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RGS4 in extracts of various cell lines using RGS4 Polyclonal Antibody (Product # PA5-95929) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RGS4 in extracts of various cell lines using RGS4 Polyclonal Antibody (Product # PA5-95929) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RGS4 was performed in L929 cells using RGS4 Polyclonal Antibody (Product # PA5-95929) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RGS4 was performed in C6 cells using RGS4 Polyclonal Antibody (Product # PA5-95929) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RGS4 was performed in L929 cells using RGS4 Polyclonal Antibody (Product # PA5-95929) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RGS4 was performed in U-2 OS cells using RGS4 Polyclonal Antibody (Product # PA5-95929) at a dilution of 1:100. Blue: DAPI for nuclear staining.
