MAB6686

antibody from R&D Systems

Targeting: MYOG bHLHc3, MYF4

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6686

Provider

R&D Systems

Product name

Human/Mouse Myogenin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Myogenin in direct ELISAs.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P15173


Isotype

IgG

Antibody clone number

671038

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations.

Dmitrieva RI, Lelyavina TA, Komarova MY, Galenko VL, Ivanova OA, Tikanova PA, Khromova NV, Golovkin AS, Bortsova MA, Sergushichev A, Sitnikova MY, Kostareva AA
Stem cells international 2019;2019:5690345

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Myogenin in C2C12 Mouse Myoblast Cells. Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cells using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB6686) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to select differentiating nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.