PA5-27755

antibody from Invitrogen Antibodies

Targeting: ESRRG ERR-gamma, ERRg, NR3B3

Antibody data

Submit

Validation data

ESRRG protein structure - PA5-27755 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ERR gamma using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ERR gamma polyclonal antibody (Product # PA5-27755) at a dilution of 1:3000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-ESRRG Polyclonal Antibody (Product # PA5-27755) and a ~57 kDa band corresponding to Estrogen-related receptor gamma was observed across cell lines and tissues tested. Nuclear enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with Retinoic Acid (Lane 2) and Mouse Brain (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ESRRG Polyclonal Antibody (Product # PA5-27755). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. ESRRG Polyclonal Antibody (Product # PA5-27755) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ESRRG Polyclonal Antibody detects ESRRG protein by Western blot analysis. A. 50 µg rat spleen lysate/extract.10 % SDS-PAGE. ESRRG Polyclonal Antibody (Product # PA5-27755) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ESRRG Polyclonal Antibody (Product # PA5-27755). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ESRRG Polyclonal Antibody (Product # PA5-27755) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.