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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- MAB79671 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Phospho-RSK1 (S380) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human, mouse, and rat RSK1 when phosphorylated at S380 in Western blots. Expected to detect RSK2, RSK3, and RSK4 phosphorylated at S386, S377, and S389, respectively, based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q15418- Isotype
- IgG
- Antibody clone number
- 1024A
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-RSK1 (S380) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S380) at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-RSK1 (S380) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, and loaded at 0.2 mg/mL. A specific band was detected for Phospho-RSK1 (S380) at approximately 90 kDa (as indicated) using 1 μg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RSK1 in HeLa Human Cell Line. RSK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line, unstimulated (panels B and D) or stimulated with PMA (panels A and C), using Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) at 25 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, upper panels; Catalog # NL004) and counterstained with DAPI (blue, lower panels). Specific staining was localized to plasma membrances and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
