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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
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- Product number
- NB100-592 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-592, RRID:AB_10001108
- Product name
- Rabbit Polyclonal ROCK2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Shroom2 regulates contractility to control endothelial morphogenesis.
ROCK2 associates with lectin-like oxidized LDL receptor-1 and mediates oxidized LDL-induced IL-8 production.
Farber MJ, Rizaldy R, Hildebrand JD
Molecular biology of the cell 2011 Mar 15;22(6):795-805
Molecular biology of the cell 2011 Mar 15;22(6):795-805
ROCK2 associates with lectin-like oxidized LDL receptor-1 and mediates oxidized LDL-induced IL-8 production.
Mattaliano MD, Wooters J, Shih HH, Paulsen JE
American journal of physiology. Cell physiology 2010 May;298(5):C1180-7
American journal of physiology. Cell physiology 2010 May;298(5):C1180-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROCK2 Antibody [NB100-592] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROCK2 Antibody [NB100-592] - Detection of Human Rho Kinase Alpha on HeLa whole cell lysate using NB100-592. B) Rabbit anti-Rho Kinase Alpha antibodies NB100-592 and NB100-593 were used to immunoprecipitate Rho Kinase Alpha.
