TA505872

antibody from OriGene

Targeting: MAPK4 Erk3-related, Erk4, PRKM4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505872

Provider

OriGene

Proper citation

OriGene Cat#TA505872, RRID:AB_2623438

Product name

MAPK4 mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)

Antibody type

Monoclonal

Description

MAPK4 mouse monoclonal antibody, clone OTI2F4 (formerly 2F4)

Host

Mouse

Conjugate

Unconjugated

Epitope

MAPK4

Isotype

IgG

Antibody clone number

OTI2F4

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK4 (RC207414, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK4.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MAPK4 mouse monoclonal antibody (TA505872) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK4(RC207414).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505872)

Validation comment

IHC