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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
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- Product number
- MA5-14903 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TrkB Monoclonal Antibody (J.977.7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Antibody clone number
- J.977.7
- Concentration
- 10 µg/mL
Submitted references Sodium-hydrogen exchanger 6 (NHE6) deficiency leads to hearing loss, via reduced endosomal signalling through the BDNF/Trk pathway.
An Extract from Shrimp Processing By-Products Protects SH-SY5Y Cells from Neurotoxicity Induced by Aβ(25-35).
Kucharava K, Brand Y, Albano G, Sekulic-Jablanovic M, Glutz A, Xian X, Herz J, Bodmer D, Fuster DG, Petkovic V
Scientific reports 2020 Feb 27;10(1):3609
Scientific reports 2020 Feb 27;10(1):3609
An Extract from Shrimp Processing By-Products Protects SH-SY5Y Cells from Neurotoxicity Induced by Aβ(25-35).
Zhang Y, Jiao G, Song C, Gu S, Brown RE, Zhang J, Zhang P, Gagnon J, Locke S, Stefanova R, Pelletier C, Zhang Y, Lu H
Marine drugs 2017 Mar 22;15(3)
Marine drugs 2017 Mar 22;15(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TrkB in extracts from rat brain and NIH/3T3/TrkB cells using TrkB monoclonal antibody (Product # MA5-14903).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-TrkB Monoclonal Antibody(J.977.7) (Product # MA5-14903) and a 90 kDa band corresponding to TrkB was observed across the tissue extracts except mouse ovary and rat ovary along with uncharacterized bands (*) around 30 kDa. Tissue extracts (30 µg lysate) of Mouse Cerebellum (Lane 1), Rat Cerebellum (Lane 2), Mouse Ovary (Lane 3), Rat Ovary (Lane 4), Mouse Brain (Lane 5) and Rat Brain(Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
