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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-17038 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CaMKIV Monoclonal Antibody (8C5B8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17038 targets CAMK4 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 8C5B8
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CAMK4 using a CAMK4 monoclonal antibody (Product # MA5-17038) against a human CAMK4 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of Jurkat (Lane 1), MOLT-4 (Lane 2), SH-SY5Y (Lane 3) and tissue extract of Rat Brain (Lane 4). The blot was probed with Anti-CaMKIV Monoclonal Antibody (Product # MA5-17038, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 52 kDa band corresponding to CaMKIV was observed across the cell lines and tissue tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CAMK4 using a CAMK4 monoclonal antibody (Product # MA5-17038) against a human CAMK4 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CAMK4 using CAMK4 monoclonal antibody (Product # MA5-17038) in HEK293 (1) and CAMK4 (AA: 35-292) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CAMK4 using CAMK4 monoclonal antibody (Product # MA5-17038) in Jurkat (1), SK-N-SH (2), Raji (3), and HeLa (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of CaMKIV was achieved by transfecting SHSY5Y with CaMKIV specific siRNAs (Silencer® select Product # s2355). Western blot analysis (Fig. a) was performed using whole cell extracts from the CaMKIV knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with CaMKIV Monoclonal Antibody (8C5B8) (Product # MA5-17038, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Reduction of signal upon siRNA mediated knock down confirms that antibody is specific to CaMKIV.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using CAMK4 monoclonal antibody (Product # MA5-17038) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CAMK4 monoclonal antibody (Product # MA5-17038) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CAMK4 monoclonal antibody (Product # MA5-17038) followed with DAB staining.
