H00005603-M13

antibody from Abnova Corporation

Targeting: MAPK13 p38delta, PRKM13, SAPK4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005603-M13

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005603-M13, RRID:AB_1679450

Product name

MAPK13 monoclonal antibody (M13), clone 3H7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant MAPK13.

Antigen sequence

MSLIRKKGFYKQDVNKTAWELPKTYVSPTHVGSGA
YGSVCSAIDKRSGEKVAIKKLSRPFQSEIFAKRAY
RELLLLKHMQHENVIGLLDVFTPASSLRNFYDFYL
VMPFMQTDLQKIMGMEFSEEKIQYLVYQMLKGLKY
IHSAGVVHRDLKPGNLAVNEDCELKILDFGLARHA
DAEMTGYVVTRWYRAPEVILSWMHYNQTVDIWSVG
CIMAEMLTGKTLFKGKDYLDQLTQILKVTGVPGTE
FVQKLNDKAAKSYIQSLPQTPRKDFTQLFPRASPQ
AADLLEKMLELDVDKRLTAAQALTHPFFEPFRDPE
EETEAQQPFDDSLEHEKLTVDEWKQHIYKEIVNFS
PIARKDSRRRSGMKL


Isotype

IgG

Antibody clone number

3H7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAPK13 expression in transfected 293T cell line by MAPK13 monoclonal antibody (M13), clone 3H7.Lane 1: MAPK13 transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MAPK13 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of MAPK13 transfected lysate using anti-MAPK13 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MAPK13 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol