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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [2]
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Validation data
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- Product number
- TA801144 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA801144, RRID:AB_2625819
- Product name
- ALK rat monoclonal antibody, clone OTI3E7 (formerly 3E7)
- Antibody type
- Monoclonal
- Description
- ALK rat monoclonal antibody, clone OTI3E7 (formerly 3E7)
- Host
- Rat
- Conjugate
- Unconjugated
- Epitope
- ALK
- Isotype
- IgG
- Antibody clone number
- OTI3E7
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ALK Luminex with 15B2 Capture (TA801288) and 3E7 Detection (TA801144) Antibodies. Substrate used: full length HEK293 cells expressed recombinant ALK protein (TP322485).
- Validation comment
- LMNX
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ALK Luminex with 14E4 Capture (TA801412) and 3E7 Detection (TA801144) Antibodies. Substrate used: full length HEK293 cells expressed recombinant ALK protein (TP322485).
- Validation comment
- LMNX
