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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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- Product number
- V2565 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Histone Antibody
- Antibody type
- Monoclonal
- Antigen
- Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- AE-4
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Histone antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Eosinophil extracellular trap cell death-derived DNA traps: Their presence in secretions and functional attributes.
Multifunctional effects of honokiol as an anti-inflammatory and anti-cancer drug in human oral squamous cancer cells and xenograft.
Novel structurally similar chromene derivatives with opposing effects on p53 and apoptosis mechanisms in colorectal HCT116 cancer cells.
Ubiquitin-specific protease 22 (USP22) positively regulates RCAN1 protein levels through RCAN1 de-ubiquitination.
Protein phosphatase-1 is involved in the maintenance of normal homeostasis and in UVA irradiation-induced pathological alterations in HaCaT cells and in mouse skin.
LMP1-Induced Sumoylation Influences the Maintenance of Epstein-Barr Virus Latency through KAP1.
Ricinine: a pyridone alkaloid from Ricinus communis that activates the Wnt signaling pathway through casein kinase 1α.
NADPH oxidase promotes neutrophil extracellular trap formation in pulmonary aspergillosis.
A nuclear export signal and oxidative stress regulate ShcD subcellular localisation: a potential role for ShcD in the nucleus.
The mechanisms by which pardaxin, a natural cationic antimicrobial peptide, targets the endoplasmic reticulum and induces c-FOS.
Ueki S, Konno Y, Takeda M, Moritoki Y, Hirokawa M, Matsuwaki Y, Honda K, Ohta N, Yamamoto S, Takagi Y, Wada A, Weller PF
The Journal of allergy and clinical immunology 2016 Jan;137(1):258-67
The Journal of allergy and clinical immunology 2016 Jan;137(1):258-67
Multifunctional effects of honokiol as an anti-inflammatory and anti-cancer drug in human oral squamous cancer cells and xenograft.
Cho JH, Jeon YJ, Park SM, Shin JC, Lee TH, Jung S, Park H, Ryu J, Chen H, Dong Z, Shim JH, Chae JI
Biomaterials 2015;53:274-84
Biomaterials 2015;53:274-84
Novel structurally similar chromene derivatives with opposing effects on p53 and apoptosis mechanisms in colorectal HCT116 cancer cells.
Lima CF, Costa M, Proença MF, Pereira-Wilson C
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2015 May 25;72:34-45
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2015 May 25;72:34-45
Ubiquitin-specific protease 22 (USP22) positively regulates RCAN1 protein levels through RCAN1 de-ubiquitination.
Hong A, Lee JE, Chung KC
Journal of cellular physiology 2015 Jul;230(7):1651-60
Journal of cellular physiology 2015 Jul;230(7):1651-60
Protein phosphatase-1 is involved in the maintenance of normal homeostasis and in UVA irradiation-induced pathological alterations in HaCaT cells and in mouse skin.
Dedinszki D, Sipos A, Kiss A, Bátori R, Kónya Z, Virág L, Erdődi F, Lontay B
Biochimica et biophysica acta 2015 Jan;1852(1):22-33
Biochimica et biophysica acta 2015 Jan;1852(1):22-33
LMP1-Induced Sumoylation Influences the Maintenance of Epstein-Barr Virus Latency through KAP1.
Bentz GL, Moss CR 2nd, Whitehurst CB, Moody CA, Pagano JS
Journal of virology 2015 Aug;89(15):7465-77
Journal of virology 2015 Aug;89(15):7465-77
Ricinine: a pyridone alkaloid from Ricinus communis that activates the Wnt signaling pathway through casein kinase 1α.
Ohishi K, Toume K, Arai MA, Sadhu SK, Ahmed F, Mizoguchi T, Itoh M, Ishibashi M
Bioorganic & medicinal chemistry 2014 Sep 1;22(17):4597-601
Bioorganic & medicinal chemistry 2014 Sep 1;22(17):4597-601
NADPH oxidase promotes neutrophil extracellular trap formation in pulmonary aspergillosis.
Röhm M, Grimm MJ, D'Auria AC, Almyroudis NG, Segal BH, Urban CF
Infection and immunity 2014 May;82(5):1766-77
Infection and immunity 2014 May;82(5):1766-77
A nuclear export signal and oxidative stress regulate ShcD subcellular localisation: a potential role for ShcD in the nucleus.
Ahmed SB, Prigent SA
Cellular signalling 2014 Jan;26(1):32-40
Cellular signalling 2014 Jan;26(1):32-40
The mechanisms by which pardaxin, a natural cationic antimicrobial peptide, targets the endoplasmic reticulum and induces c-FOS.
Ting CH, Huang HN, Huang TC, Wu CJ, Chen JY
Biomaterials 2014 Apr;35(11):3627-40
Biomaterials 2014 Apr;35(11):3627-40
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of HeLa cell lysate (nuclear fraction) with Histone antibody (clone AE-4). Observed molecular weight ~22/30-33kDa (unmodified/modified).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Histone H1 antibody (AE-4)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (AE-4)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: FFPE human angiosarcoma tested with Histone antibody (AE-4)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: FFPE human testicular carcinoma tested with Histone antibody (AE-4)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC: FFPE human pancreas tested with Histone antibody (AE-4)
