Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB2177 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal GITR Ligand/TNFSF18 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse GITR Ligand/TNFSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human GITR Ligand, recombinant mouse (rm) CD40, or rmTNF-alpha is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.
Mahmud SA, Manlove LS, Schmitz HM, Xing Y, Wang Y, Owen DL, Schenkel JM, Boomer JS, Green JM, Yagita H, Chi H, Hogquist KA, Farrar MA
Nature immunology 2014 May;15(5):473-81
Nature immunology 2014 May;15(5):473-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by GITR Ligand/TNFSF18 and Neutralization by Mouse GITR Ligand/TNFSF18 Antibody. In the presence of Mouse CD3 epsilon Monoclonal Anti-body (0.5 µg/mL, Catalog # MAB484) and a cross-linking antibody, polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (Catalog # 2177-GL) stimulates proliferation in mouse CD4+ T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/ TNFSF18 Monoclonal Antibody (Catalog # MAB2177). The ND50 is typically 2-8 µg/mL.