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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-235-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGF-4 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human FGF-4 in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross-reactivity with recombinant mouse FGF-4 is observed, and less than 15% cross-reactivity with recombinant human (rh) FGF-6 is observed. It will not neutralize the biological activity of bovine FGF acidic, rhFGF acidic, bovine FGF basic, rhFGF basic, rhFGF-5, rhFGF-6, or rh beta-ECGF.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08620- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by FGF-4 and Neutralization by Human FGF-4 Antibody. Recombinant Human FGF-4 (Catalog # 235-F4) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-4 Polyclonal Antibody (Catalog # AB-235-NA). The ND50 is typically 6-9 µg/mL.
