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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- PA1-28931 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNAI2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA1-28931 detects GNAI2 from human samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GNAI2 in transfected HEK293 cells (left lane). Samples were probed with a GNAI2 polyclonal antibody (Product # PA1-28931).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human GNAI2 detected by Western blot in transfected HEK293 cells (left lane) using GNAI2 Polyclonal Antibody (Product # PA1-28931).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human GNAI2 detected by Western blot in transfected HEK293 cells (left lane) using GNAI2 Polyclonal Antibody (Product # PA1-28931).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GNAI2 Polyclonal Antibody (Product # PA1-28931) and a 40kDa band corresponding to GNAI2 was observed across cell lines and tissues tested. Membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), U-937 (Lane 2), U-87 MG (Lane 3), Jurkat (Lane 4), Mouse Brain (Lane 5) and Rat Brain (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GNAI2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GNAI2 Polyclonal Antibody (Product # PA1-28931) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to the cytoplasm and nucleus. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
