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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-33684 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33684, RRID:AB_10004096
- Product name
- Rabbit Polyclonal Annexin A1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A1 Antibody [NBP1-33684] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE; antibody diluted at 1:10000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A1 Antibody [NBP1-33684] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A1 Antibody [NBP1-33684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A1 Antibody [NBP1-33684] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Annexin A1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A1 Antibody [NBP1-33684] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Annexin A1 Antibody [NBP1-33684] - Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 ug of Annexin A1 antibody. Western blot analysis was performed using Annexin A1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-33684] - Paraffin-embedded N87 Xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin A1 Antibody [NBP1-33684] - Paraffin-embedded mouse lung. Annexin A1 antibody dilution: 1:500.
