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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA500915 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500915, RRID:AB_11123792
- Product name
- Anti-RALBP1 (RIP1) mouse monoclonal antibody, clone OTI6G10 (formerly 6G10)
- Antibody type
- Monoclonal
- Description
- Anti-RALBP1 (RIP1) mouse monoclonal antibody, clone OTI6G10 (formerly 6G10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RALBP1
- Isotype
- IgG
- Antibody clone number
- OTI6G10
- Vial size
- 100 µl
- Concentration
- 0.9 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (RC201524, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RALBP1 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RALBP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-RALBP1 mouse monoclonal antibody (TA500915) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1(RC201524).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of RALBP1 by using TrueMab monoclonal anti-RALBP1 antibodies (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate),
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-RALBP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500915, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-RALBP1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500915, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY RALBP1(RC201524)(Red) or empty vector control plasmid(Blue) were immunostained with anti-RALBP1 mouse monoclonal(TA500915), and then analyzed by flow cytometry.
- Validation comment
- FC
