Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-28382 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2.
- Concentration
- 1.18 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WNK1 using 30 µg of H1299 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a WNK1 polyclonal antibody (Product # PA5-28382) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WNK1 was performed by separating 30 µg of whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNK1 Polyclonal Antibody (Product # PA5-28382) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WNK1 Polyclonal Antibody (Product # PA5-28382). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 Polyclonal Antibody (Product # PA5-28382) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WNK1 was performed by separating 30 µg of whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNK1 Polyclonal Antibody (Product # PA5-28382) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of WNK1 in methanol-fixed HeLa cells using a WNK1 polyclonal antibody (Product # PA5-28382) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of WNK1 was performed in paraffin-embedded human lung adenocarcinoma tissue using WNK1 Polyclonal Antibody (Product # PA5-28382) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using WNK1 (Product # PA5-28382) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WNK1 Polyclonal Antibody detects WNK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. WNK1 stained by WNK1 Polyclonal Antibody (Product # PA5-28382) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.