- PA5-27323 - Invitrogen Antibodies VCP antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of VCP using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VCP polyclonal antibody (Product # PA5-27323) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of VCP using 30 µg of A) A431 and B) H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VCP polyclonal antibody (Product # PA5-27323) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using VCP Polyclonal Antibody (Product # PA5-27323). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. VCP Polyclonal Antibody (Product # PA5-27323) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using VCP Polyclonal Antibody (Product # PA5-27323). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VCP Polyclonal Antibody (Product # PA5-27323) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: VCP Polyclonal Antibody detects VCP protein by western blot analysis. A. 50 µg Rat brain lysate/extract.7.5% SDS-PAGE. VCP Polyclonal Antibody (Product # PA5-27323) dilution: 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of VCP was achieved by transfecting MCF7 cells with VCP specific siRNAs (Silencer® select Product # s14765). Western blot analysis (Fig. a) was performed using whole cell extracts from the VCP knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with VCP Polyclonal Antibody (Product # PA5-27323, 1:8000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to VCP.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extract (30 µg lysate) of SKOV3 (Lane 1), MDA-MB-231 (Lane 2), MCF7 (Lane 3), GTL16 (Lane 4), HCT116 (Lane 5), PC3 (Lane 6), A431 (Lane 7), PANC-1 (Lane 8) and A549 (Lane 9). The blot was probed with Anti-VCP Polyclonal Antibody (Product # PA5-27323, 1:8000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 97 kDa band corresponding to VCP was observed in all cell lines.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: VCP Polyclonal Antibody detects VCP protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: VCP protein stained by VCP Polyclonal Antibody (Product # PA5-27323) diluted at 1:500. Blue: Hoechst 33343 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry (Frozen) analysis of VCP was performed in frozen-sectioned adult mouse cerebellum tissue using VCP Polyclonal Antibody (Product # PA5-27323) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human breast cancer, using VCP (Product # PA5-27323) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-27323